In this work we present the development of a large set of single nucleotide polymorphisms (SNPs) discovered in two populations of bighorn sheep (Ovis canadensis). To do so we used restriction-site associated DNA (RAD) sequencing of four individuals from each population. Through alignment of reads to the domestic sheep (Ovis aries) genome we discovered >83,000 SNPs, of which >38,000 are suitable for assays such as an Illumina SNP chip. These loci will allow for fine-mapping of loci associated with horn size, and examination of the consequences of the genetic rescue including mapping genes underling differences in life-history characteristics.
Usage Notes:
Miller et al VCF SNP genotypes
Miller et al VCF SNPs.xlsx
Miller et al BAM File
Sequence alignments (one .bam file per individual) of Illumnia reads to the domestic sheep genome.
MillerBighornBAMfiles.zip
Miller et al Raw Read
Sequence files (one per individual) in .fastq format.
MillerBighornIllumniaReads.zip
Miller et al VCF SNPs
RAD Loci and genotypes for 8 bighorn sheep