Time-course full profiling of circulating miRNAs in neurologically deceased organ donors: a proof of concept study to understand the onset of the cytokine storm

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Description:
Neurologically deceased organ donors (NDDs) generally display an immune response involving an intense production of pro-inflammatory cytokines referred to as the cytokine storm. The sudden surge of inflammatory mediators in circulation promotes tissue and organ damages and ultimately leads to poor transplant outcome. As microRNAs (miRNAs) are frequently proposed as key regulators of inflammation and are relatively stable in circulation, changes in their profiles could play a role in the onset of the cytokine storm in NDDs. In this proof-of-concept study, we sought to investigate differentially abundant circulating miRNAs in a temporal manner between neurological death and organ recovery and to assess the association between specific miRNAs and levels of inflammatory cytokines in blood. Plasma samples from five NDDs were obtained at multiple time points between organ donation consent and organ recovery. Using a time-course analysis and miRNA sequencing, we identified 32 plasma miRNAs fluctuating between consent and organ recovery (false discovery rate; q-value < 0.1). Eleven miRNAs relatively abundant (>100 reads) and detected in all samples were selected for further biological pathway analysis (miR-486-3p, miR-103a-3p, miR-106b-3p, miR-182-5p, miR-101-3p, miR-10a-5p, miR-125a-5p, miR-146b-5p, miR-26a-5p, miR-423-5p, miR-92b-3p). These miRNAs targeted genes such as c-JUN (TNF signalling pathway) and eEF2 (AMPK pathway), suggesting a potential role in regulation of inflammation. Our results contribute to a better understanding of the miRNAs dynamic after neurological death in organ donors and could potentially be used to predict the related early cytokine storm.Trial registration: ClinicalTrials.gov ID NCT03786991. Registered December 2018
Author(s):
Clément, Andrée-Anne

Clément, Andrée-Anne

Université de Sherbrooke
, Lamarche, Daphnée

Lamarche, Daphnée

, Masse, Marie-Hélène

Masse, Marie-Hélène

Université de Sherbrooke
, Légaré, Cécilia

Légaré, Cécilia

Université de Sherbrooke
, Tai, Lee-Hwa

Tai, Lee-Hwa

, Deland, Laurence Fleury

Deland, Laurence Fleury

, Battista, Marie-Claude

Battista, Marie-Claude

Université de Sherbrooke
, Bouchard, Luigi

Bouchard, Luigi

Université de Sherbrooke Centre Hospitalier Universitaire de Sherbrooke Centre Intégré Universitaire de Santé et de Services Sociaux du Saguenay–Lac-Saint-Jean
, and D’Aragon, Frédérick

D’Aragon, Frédérick

Université de Sherbrooke Centre Hospitalier Universitaire de Sherbrooke
Source Repository:
Figshare
Publisher(s):
Taylor & Francis
License:
Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode
URL:
https://tandf.figshare.com/articles/dataset/Time-course_full_profiling_of_circulating_miRNAs_in_neurologically_deceased_organ_donors_a_proof_of_concept_study_to_understand_the_onset_of_the_cytokine_storm/19808493
Publication date:
2022
Subjects:
FOS: Biological sciences, Medicine, 69999 Biological Sciences not elsewhere classified, Cell Biology, Microbiology, Biochemistry, Genetics, Molecular Biology, Developmental Biology, FOS: Health sciences, Physiology, 110309 Infectious Diseases, Immunology, FOS: Clinical medicine, Biophysics, 29999 Physical Sciences not elsewhere classified, and FOS: Physical sciences
Identifier:
https://doi.org/10.6084/m9.figshare.19808493

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Citation

APA Citation:
Clément, A.-A., Lamarche, D., Masse, M.-H., Légaré, C., Tai, L.-H., Deland, L. F., Battista, M.-C., Bouchard, L., & D’Aragon, F. (2022). Time-course full profiling of circulating miRNAs in neurologically deceased organ donors: a proof of concept study to understand the onset of the cytokine storm [Data set]. Figshare. https://tandf.figshare.com/articles/dataset/Time-course_full_profiling_of_circulating_miRNAs_in_neurologically_deceased_organ_donors_a_proof_of_concept_study_to_understand_the_onset_of_the_cytokine_storm/19808493
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