This dataset includes greenhouse gases (GHG; CO2 and CH4) and dissolved organic matter (DOM) concentrations and composition, and water chemical and geographic (i.e., climate, vegetation cover and pool morphometry) properties of peatland pools. To evaluate the inter-regional pattern of GHG and DOM concentrations and composition, we sampled water from 73 peatland pools across five regions in eastern Canada (Grande plée Bleue, southern Quebec and Minganie region, eastern Quebec) and southern Chilean Patagonia (Punta Arenas, Karukinka Park and Navarino Island) in mid-summer 2019 (July-August in Quebec and January-February in Patagonia). We also conducted inter-annual sampling in Grande plée Bleue in 2019 and 2020 (between June and September) to identify the temporal patterns in GHG and DOM concentrations and composition. We performed and validated a 3-component PARAFAC model to identify the DOM component in peatland pool. We used this data to understand and quantify the intra- and inter-regional patterns and drivers of DOM and dissolved GHG (CO2 and CH4) in peatland pools as well as their links in contrasting geographical conditions. Inter-regional spatial data: Area (m2), Depth (cm), Elevation (m), Trees (%), Shrubs (%), Herbs (%), Mosses (%), MAT (°C), MAP (mm), pH, TN (mg/L), TP (μg/L), DOC (mg/L), Specific UV absorbance (SUVA; Lmg/Cm), Ratio of absorbance at 465 nm to absorbance at 665 nm (E4/E6), Absolute concentrations (C1-C3; RU) and relative abundance (%C1-%C3) of dissolved organic matter, CO2-C (mg/L), CH4 (μg/L), CO2:CH4 and
Temporal data: DO (mg/L), pH, TN (mg/L), TP (μg/L), NO3 (μg/L), NH4 (μg/L), PO4 (μg/L), DOC (mg/L), Specific UV absorbance (SUVA; Lmg/Cm), Absolute concentrations (C1-C3; RU) and relative abundance (%C1-%C3) of dissolved organic matter, CO2-C (mg/L), CH4 -C (μg/L), CO2:CH4