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Publication: Soil greenhouse gas emissions and grazing management in northern temperate grasslands
Contact Details: Please contact Ma Zilong at mazlong@mail.sysu.edu.cn and Bharat Shrestha at shresthabm@gmail.com if you have any further questions
Methods: Growing season GHG fluxes were measured in the field bi-weekly from mid-August to mid-October in 2017, early May to mid-October in 2018, and early May to early September in 2019 using dark static chambers (65.5 × 17 × 15.5 cm height) at six random sampling points within a representative area of each study ranch. Ambient air samples(20 mL) were collected prior to placing the chamber-lids over the static chambers (ambient condition, t = 0) and again at 10, 20, and 30 min after placing the chamber-lid over the static chamber, using an airtight syringe (Norm-Ject, Henke Sass Wolf, Tuttlingen, Germany) through a rubber septum. Samples were then stored in pre-evacuated 12 mL soda glass Isomass Exetainers (Labco Limited, Lampeter, Wales, UK) to provide a positive pressure in the Exetainer. Collected gas samples were transported in dark boxes to the Forest Soil Laboratory at the University of Alberta, Edmonton, and were analyzed using a Varian CP-3800 gas chromatograph (Varian Canada, Mississauga, Canada) equipped with a thermal conductivity detector, a flame ionization detector and an electron capture detector for determining the CO2, CH4, and N2O concentrations, respectively.