This repository contains data on environmental variables, sedimentary organic matter, benthic nutrient fluxes and macrofaunal communities collected from 10 stations along the Northwest Atlantic continental margin sampled onboard the NOAA research vessel Henry B. Bigelow in June 2017 using the ROV ROPOS. Data were used to characterize patterns and drivers of organic matter remineralization, macrofaunal diversity, community composition, and biological trait expression in contrasting habitats (shelf, slope, submarine canyons and inter-canyon areas). The dataset includes the ReadMe (metadata) text file, and 5 data sheets (.csv).