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Carvey, Quinn; Pavey, Scott A.; Diamond, Tony; Davoren, Gail K.; Lavoie, Raphael A.; Legard, Matthew; Robertson, Gregory J.; Runnells, Emily; Petalas, Christina; Major, Heather — 2024-02-20 Blood samples were collected from Atlantic Puffins breeding at five colonies in Atlantic Canada. DNA was extracted using a DNeasy Blood and Tissue DNA Kit from Qiagen. Triple digest genotype-by-sequencing (3D-GbS) library preparation was performed using three restriction enzymes (PstI/NsiI/MspI). Samples were sequenced on one lane using 150 bp, paired-end sequencing with an Illumina NovaSeq 6000. Data were assembled with Stacks v.2.6.1, see publication for full methodological details. Filtered SNP datasets used in genetic analyses are included here.