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University of New Brunswick at Saint John; Prince Edward Island Museum and Heritage Foundation; Canadian Heritage Information Network; Canadian Museum of Civilization; Gorsebrook Research Institute, St. Mary’s University; Memorial University of Newfoundland; New Brunswick Museum, Saint John; Newfoundland Museum; Nova Scotia Museum, Halifax 2017-02-12 Atlantic Canada, Prince Edward Island, Nova Scotia, New Brunswick, Newfoundland The Atlantic Canada Newspaper Survey (ACNS) project was designed to provide a machine-readable database of goods and services offered for sale in important east coast regional newspapers before 1900. This newspaper research database has as its focus nine broad categories of commodities based upon Statistics Canada’s “Trade of Canada Commodity Classification”. In the 1980s work on the database was supported by the Canadian Museum of Civilization. The project received SSHRC funding in 1986-1987 as part of its Research Tools Program. In June 2013, the Canadian Heritage Information Network was no longer able to host the database due to accessibility issues. CHIN exported the dataset and provided a copy to UPEI in the hopes that it would be useful.
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E. Don Stevens 2018-02-09 Fish are a useful animal model for research, but our improvement in some aspects of their welfare has not kept pace with their increased popularity for this use. For example, researchers rarely use analgesics. We evaluated the side effects of lidocaine, a local anesthetic widely used in human and veterinary medicine. We infiltrated lidocaine on each side of the dorsal fin (total 20 mg/kg) of young rainbow trout (15 fish per group) compared with infiltration with an equal volume of saline. We monitored behaviour of individual trout during the 4-hour trial. Food was presented 5 times during the trial (30 min, 1h, 2h, 3h, 4h after infiltration) and we analyzed behaviour for 1 minute before and after food presentation. Behaviour of Saline-Infiltrated trout compared with trout that received no infiltration showed that infiltration in and of itself had no statistically significant effects on trout behaviour. However, there were many statistically significant effects of Lidocaine-Infiltrated trout compared with Saline-Infiltrated trout; none of the side-effects were adverse.
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McEwan, Gregor; Groner, Maya; Fast, Mark; Gettinby, George; Revie, Crawford 2015-10-08 Sea Lice are a problem for farming Atlantic Salmon. They are showing resistance to common chemotherapeutants. We show that wild salmon populations can provide refugia for the sea lice and mitigate the growth of resistance.
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Department of Communities, Land and Environment 2016-05-04 Prince Edward Island This data set provides pesticide sample analyses results for finfish (brook and rainbow trout) and shellfish (mussels and soft shell clams) for the province’s Pesticide Monitoring Program. The sampling includes a total of nine rivers that are tested across PEI, with three of the rivers being sampled each year. Finfish are collected from the river by electrofishing or rod and reel. Shellfish are collected from the same river systems manually, as close to the finfish sampling as possible. The purpose of the sampling is to determine if pesticide residues accumulate in the fish/shellfish. Collected 2007-Aug-22 to 2012-Nov-15
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Department of Economic Development and Tourism 2016-05-06 Prince Edward Island Site nights available and sold by year for all the Provincial Parks Campgrounds 2005-01-01 to 2015-12-31
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Prince Edward Island Finance and Municipal Affairs, Taxation and Property Records, Geomatic Services 2000-01-01 Prince Edward Island This layer identifies the geographic area each ambulance provider responds to in PEI. Supplemental_Information: ***** Spatial Reference Information (Beg) ***** Projection Parameters The coordinate plane of the province's Geographic Information System was realized by using a Double Stereographic Projection with parameters specific to this province as listed below and is referred to as the PEI Survey Reference System. The projection is referenced to the North American Datum of 1983 realized from the Canadian Spatial Reference System (NAD83(CSRS)). The Geodetic Reference System of 1980 (GRS80) is the ellipsoid upon which the datum is realized. This reference ellipsoid or ellipse of revolution, was fixed in 1980 as a "best" means to mathematically represent the earth%u2019s surface. Parameters for the PEI Survey Reference System are as follows: Projection Stereographic Double Projection Origin Latitude: N 47° 15' Longitude: W 63° 00' Scale Factor at Origin 0.999912 False Origin Northing: 800,000 Metres Easting: 400,000 Metres NAD 83(CSRS) Datum Parameters a=6378137.0000 b=6356752.3141 1/f=298.257222101 First Eccentricity e² =0.006694380 Second Eccentricity e'² =0.006739497 Gaussian Mean Radius at Origin =6,379,782.0995 Metres PEIGeocalc is a transformation software that enables coordinates to be converted to and from various projections and datums such as NAD83(CSRS), ATS77, or again NAD27. For further information or to obtain a copy of this software contact: Chief Surveyor: Serge Bernard Spatial Information Raster: Number of Columns: Number of Rows: Pixel Resolution (m): Data Type: byte ***** Spatial Reference Information (End) *****
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Department of Communities, Land and Environment 2016-05-09 Prince Edward Island This data set provides pesticide sample analyses results for stream sediment samples for the province’s Pesticide Monitoring Program. The sampling includes sampling a total of nine rivers across PEI, with three of the rivers being sampled each year. Sediment samples are collected once in July, twice in August (once during a normal/dry weather and once during wet weather), and once in September. The two samples in July and September are collected during dry weather. Sediment sampling involves Department staff collecting sediment from the stream bed of the site, using a small shovel. Samples are then sent to a laboratory for analysis. These samples are collected in the same rivers as the Stream Water and the Finfish/Shellfish Pesticide datasets.
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Kerr, Russell G.; Duncan, Katherine; Haltli, Bradley; Gill, Krista 2014-02-13 New Brunswick, Canada Supplementary Information includes tables and figures. Abstract from the article <br/> Actinomycetes are an important resource for the discovery of natural products with therapeutic properties. Bioprospecting for actinomycetes typically proceeds without a priori knowledge of the bacterial diversity present in sampled habitats. In this study, we endeavored to determine if overall bacterial diversity in marine sediments, as determined by 16S rDNA amplicon pyrosequencing, could be correlated with culturable actinomycete diversity, and thus serve as a powerful tool in guiding future bioprospecting efforts. Overall bacterial diversity was investigated in eight marine sediments from four sites in New Brunswick, Canada, resulting in over 44,000 high quality sequences (x̄ = 5610 per sample). Analysis revealed all sites exhibited significant diversity (H’ = 5.4 to 6.7). Furthermore, statistical analysis of species level bacterial communities (D = 0.03) indicated community composition varied according to site and was strongly influenced by sediment physiochemical composition. In contrast, cultured actinomycetes (n = 466, 98.3% Streptomyces) were ubiquitously distributed among all sites and distribution was not influenced by sediment composition, suggesting that the biogeography of culturable actinomycetes does not correlate with overall bacterial diversity in the samples examined. These actinomycetes provide a resource for future secondary metabolite discovery, as exemplified by the antimicrobial activity observed from preliminary investigation.
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MacKay, Alexander H.; Fenech, Adam 2017-10-06 Nova Scotia This dataset includes data transcribed from records collected in the early 1900s. From 1900 to 1923, an influential inspector of schools in Nova Scotia, Dr. A.H. MacKay, recruited a number of knowledgeable teachers around the province to use their students to observe 100 natural occurrences each year, and report them in a standardized way. This is the science of phenology - the study of the seasonal timing of life cycle events. These observations included the appearance of blooming wildflowers, cultivated plants, migratory birds, mammals, amphibians plus the freezing of lakes and rivers, appearance of frost and snow, number and severity of thunderstorms, hurricanes, etc. In addition, the timing of human agricultural practices was also recorded, including calving, seeding, potato planting, and haying. Tracking the timing of naturally occurring events helps show trends in the effects on biota and human activities as a result of climate change and weather variability.
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Kamunde C; Sappal R; Melegy TM 2019-07-04 Canada This dataset contains results of a 30-day feeding trial testing the effect of AquaArom (a seaweed meal derived from brown seaweeds of the genus Laminaria) in Atlantic salmon smolts. Overall, the data indicate that inclusion of AquaArom in the diet of Atlantic salmon smolts increases food consumption and enhances growth performance, improves plasma and tissue antioxidant capacity, and alleviates adverse effects of temperature stress.
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Collins Kamunde 2019-12-10 High energy demand for continuous mechanical work and large number of mitochondria predispose the heart to excessive reactive oxygen species (ROS) production that may precipitate oxidative stress and heart failure. While mitochondria have been proposed as a unifying cellular target and driver of adverse effects induced by diverse stressful states, there is limited understanding of how heart mitochondrial ROS homeostasis is affected by combinations of stress factors. Thus, we probed the effect of copper (Cu) and thermal stress on ROS (as hydrogen peroxide, H2O2) emission and elucidated the effects of Cu on ROS production sites in rainbow trout heart mitochondria using the Amplex UltraRed-horseradish peroxidase detection system optimized for our model. Mitochondria oxidizing malate-glutamate or succinate were incubated at 4, 11 (control) and 23 oC and exposed to a range (1-100 μM) of Cu concentrations. We found that the rates and patterns of H2O2 emission depended on substrate type, Cu concentration and temperature. In mitochondria oxidizing malate-glutamate, Cu increased the rate of H2O2 emission with a spike at 1 μM while temperature had no effect. In contrast, both temperature and Cu increased the rate of H2O2 emission in mitochondria oxidizing succinate with a prominent spike at 25 μM Cu. The rates of H2O2 emission at the three temperatures during the spike imposed by 25 μM Cu were of the order 11 > 23 > 4 oC. Interestingly, 5 μM Cu supressed H2O2 emission in mitochondria oxidizing succinate or malate-glutamate suggesting a common mechanism of action independent of substrate type. In the absence of Cu, the site-specific capacities of H2O2 emission were: complex III outer ubiquinone binding site (site IIIQo) > complex II flavin site (site IIF) ≥ complex I flavin site (site IF) > complex I ubiquinone-binding site (site IQ). Rotenone marginally increased succinate-driven H2O2 emission suggesting either the absence of reverse electron transport (RET)-driven ROS production at site IQ or masking of the expected rotenone response (reduction) by H2O2 produced from other sites. Cu acted at multiple sites in the electron transport system resulting in different site-specific H2O2 emission responses depending on the concentration. Specifically, site IF H2O2 emission was suppressed by Cu concentration-dependently while H2O2 emission by site IIF was inhibited and stimulated by low and high concentrations of Cu, respectively. Additionally, emission from site IIIQo was stimulated by low and inhibited by high Cu concentrations. Overall, our study unveiled distinctive effects and sites of modulation of mitochondrial ROS production by Cu with implications for cardiac redox signaling networks and development of mitochondria-targeted Cu-based drugs.
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Onukwufor, JO; Stevens, D; Kamunde, C 2016-08-10 The mitochondrial ATP-sensitive K+ (mitoKATP) channel plays an important role in mitochondrial physiology. We studied its role in protecting against hypoxia–reoxygenation (H-R)-induced mitochondrial dysfunction in fish. We used two modulators of the channel, diazoxide and 5-hydroxydecanoate (5-HD), and tested the effects of low and high doses on mitochondrial bioenergetics and volume under normoxia and after H-R using buffers with and without magnesium and ATP (Mg-ATP). Overall, we showed that in the presence of Mg-ATP, both opening of mitoKATP channels and bioenergetic effects of diazoxide were protective against H-R in fish liver mitochondria, whereas in the absence of Mg-ATP only the bioenergetic effect of diazoxide was protective.

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