Search

Dryad Logo
Data from: DNA metabarcoding improves the taxonomical resolution of visually determined diet composition of beaked redfish (Sebastes sp.)
Brown-Vuillemin, Sarah; Bernatchez, Louis; Normandeau, Eric; Hernandez, Cécilia; Chabot, Denis; Tremblay, Réjean; Sirois, Pascal; Nozères, Claude; Robert, Dominique — 2022-12-05 <p class="pf0" style="text-align:left;"><span lang="EN-CA">Beaked r</span><span lang="EN-CA">edfish, dominated by <em>Sebastes mentella</em>, have recently reached record abundance levels in the Gulf of St. Lawrence (GSL) and knowledge of their diet composition is essential to understand the trophic role that these groundfish play in the ecosystem. The objective of the present study was to compare the performance of the visual examination and DNA metabarcoding of stomach contents of the same individual redfish caught in the estuary and northern Gulf of St. Lawrence. Using a universal metazoan mitochondrial cytochrome c oxidase subunit I (COI) marker, a total of 27 taxonomic sequence matches, 16 at the species level considered as primary prey, were obtained from 185 stomachs with DNA metabarcoding and compared to </span><span lang="EN-GB">the</span> <span lang="EN-CA">26 prey types, 16 at genus or species level, obtained with stomach content analysis (SCA). While both techniques pointed to a similar definition of diet composition, our results</span><span lang="EN-CA"> also revealed that the SCA and DNA metabarcoding perform differently among prey categories, both in terms of detectability and taxonomical resolution, as well as in estimated contribution to diet. </span><span lang="EN-US">The use of DNA metabarcoding along with SCA improves the taxonomical resolution of visually determined prey,</span><span lang="EN-CA"> which supports the concept that both techniques provide useful complementary information that is best used together to gain a maximum level of information on the predator’s diet.</span></p>
PANGAEA Logo
Seawater carbonate chemistry and survival, metabolic traits and enzyme activities of multiple populations of the northern shrimp (Pandalus borealis)
PANGAEA
Guscelli, Ella; Noisette, Fanny; Chabot, Denis; Blier, Pierre U; Hansen, Tanya; Cassista-Da Ros, Manon; Pepin, Pierre; Skanes, Katherine R; Calosi, Piero — 2024 This study aimed at determining the physiological responses of the northern shrimp, Pandalus borealis, at different levels of biological organization and from four different geographic origins, exposed to elevated temperature and low pH to define its sensitivity to future ocean warming and acidification. Shrimp sampled within the northwest Atlantic were exposed for 30 days to combinations of three temperature (2, 6 or 10°C) and two pH levels (7.75 or 7.40). Survival, metabolic rates, whole-organism aerobic performance and cellular energetic capacity were assessed at the end of the exposure.This dataset is included in the OA-ICC data compilation maintained in the framework of the IAEA Ocean Acidification International Coordination Centre (see https://oa-icc.ipsl.fr). Original data were downloaded from the PANGAEA dataset (see Source). In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2024) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2024-05-13.This project was further supported by:- Strategic Program for Ecosystem- Based Research and Advice grant (SPERA)- Aquatic Climate Change Adaptation Services Program grant (ACCASP)- MITACS-Ouranos Accelerate grant- Réal-Decoste Ouranos scholarship, Award no. 286109 https://creativecommons.org/licenses/by/4.0/legalcode
PANGAEA Logo
Metabolomics reprogramming of the northern shrimp exposed to combined ocean change drivers
PANGAEA
Guscelli, Ella; Chabot, Denis; Vermandele, Fanny; Madeira, Diana; Calosi, Piero — 2023 The dataset contains information on the metabolomics reprogramming of the northern shrimp Pandalus borealis collected from four distinct geographic origins (i.e. St. Lawrence Estuary (SLE) 48° 35' N, 68° 35' W, May 2018 ; Eastern Scotian Shelf (ESS), 45° 23' N, 61° 04' W, February 2019 ; Esquiman Channel (EC), 50° 44' N, 57°29' W, July 2019 ; and Northeast Newfoundland Coast (NNC), 50° 18' N, 54° 16' W, November 2019) and exposed for 30 days under laboratory conditions to different ocean global change scenarios of temperature (2, 6, and 10 °C) and pH (7.75 and 7.40), in isolation and in combination. The dataset contains the concentration of key metabolites linked to the aerobic and anaerobic metabolism, expressed as ng metabolite per mg wet weight. Metabolite extraction, identification and quantification was carried out on shrimp muscle tissue by Les laboratoires Iso-BioKem Inc. in February/March 2021. This project was further supported by:1: The DFO Strategic Program for Ecosystem-Based Research and Advice2.The Aquatic Climate Change Adaptation Services Program3. FIR UQAR4. MITACS-Ouranos Accelerate5. The Canada Foundation for Innovation (CFI)6. Réal-Decoste Ouranos scholarship (286109) https://creativecommons.org/licenses/by/4.0/legalcode

Map search instructions

1.Turn on the map filter by clicking the “Limit by map area” toggle.
2.Move the map to display your area of interest. Holding the shift key and clicking to draw a box allows for zooming in on a specific area. Search results change as the map moves.
3.Access a record by clicking on an item in the search results or by clicking on a location pin and the linked record title.
Note: Clusters are intended to provide a visual preview of data location. Because there is a maximum of 50 records displayed on the map, they may not be a completely accurate reflection of the total number of search results.

(Author: Chabot, Denis)

Limit by:

Clear all

to