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Impacts of the Husky oil spill on gut microbiota of native fish species to the North Saskatchewan River
Federated Research Data Repository / dépôt fédéré de données de recherche
DeBofsky, Abigail; Xie, Yuwei; Jardine, Timothy D.; Jones, Paul; Giesy, John — 2024-03-27 These data are collected from goldeye (Hiodon alosoides), walleye (Sander vitreus), northern pike (Esox lucius), and shorthead redhorse (Moxostoma macrolepidotum) from the North Saskatchewan River in 2017, following the Husky oil spill. The samples (gut contents) were sterilely collected from the guts of each fish and stored at -80C until DNA extraction. The guts were used for 16S rRNA sequencing to analyze differences in the microbiome among species as well as the impact of the Husky oil spill on gut microbial communities. Each fish was analyzed independently (samples were not pooled). The results of this study showed a significant effect of host species on the gut microbiome as well as marginal effects of the Husky oil spill. https://creativecommons.org/licenses/by/4.0/
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The impact of fluoxetine on the gut microbiome of Pimephales promelas
Federated Research Data Repository / dépôt fédéré de données de recherche
Weber, Alana; Xie, Yuwei; Challis, Jonathan; DeBofsky, Abigail; Ankley, Phillip; Hecker, Markus; Jones, Paul; Giesy, John — 2024-03-27 This data is supplementary information regarding the effects of fluoxetine on the gut microbiome of Fathead Minnows when aqueously exposed to the compound. After a 28-day exposure to three different concentrations of fluoxetine; 0.01, 10 and 100 µg/L, or control (0 µg/L), fish were euthanized, and gut contents was collected. Using DNA extraction methods, PCR amplification and sequencing, bioinformatics was then performed to interpret the metagenomic data. All tests and data were collected between September 2019 and January 2021 at the University of Saskatchewan, Saskatoon, SK, Canada. This data was generated to better understand how pharmaceuticals found downstream of wastewater treatment plants may impact the gut microbiome of aquatic organisms. https://creativecommons.org/licenses/by/4.0/

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(Author: Jones, Paul)

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