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Supplemental data: Perceived producer barriers to the implementation of best management practices for the early detection of mild lameness and selective antibiotic therapies for nonsevere clinical mastitis and at drying-off in dairy cattle
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Brunt, Michael; Ritter, Caroline; Renaud, David L.; LeBlanc, Stephen; Kelton, David F. — 2024-11-07 A total of 6 focus groups involving 35 participants from 30 farms were held in Ontario Canada from February 5, to March 8, 2024. Twenty-five (71%) individuals who identified as a man and ten (29%) who identified as a woman. Participants were on average 39 years old (median = 37, range = 20 to 80). Twenty-three of the farms (77%) were freestall and seven (23%) were tiestall. Sand bedding was used by 14 farms (47%) and combinations of shavings, straw and mats were used by 16 farms (53%). Farms had an average of 105 lactating cows (median = 82, range = 40 to 425). Ten farms (33%) milked their cows in a parlor, 13 (43%) used an automatic milking systems, and seven (24%) farms used other milking systems.
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Effects of parity, blood progesterone, and non-steroidal anti-inflammatory treatment on the dynamics of the uterine microbiota of healthy postpartum dairy cows
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Pascottini, Osvaldo Bogado; Weese, J. Scott; LeBlanc, Stephen — 2020-12-17 This study evaluated the effects of treatment with meloxicam (a non-steroidal anti-inflammatory drug), parity, and blood progesterone concentration on the dynamics of the uterine microbiota of 16 clinically healthy postpartum dairy cows. Seven primiparous and 9 multiparous postpartum Holstein cows either received meloxicam (0.5 mg/kg SC, n = 7 cows) once daily for 4 days (10 to 13 days in milk (DIM)) or were untreated (n = 9 cows). Endometrial cytology samples were collected by cytobrush at 10, 21, and 35 DIM, from which the microbiota analysis was conducted using 16S rRNA gene sequence analysis. A radioimmunoassay was used to measure progesterone concentration in blood serum samples at 35 DIM and cows were classified as ˃ 1 ng/mL (n = 10) or ≤ 1 ng/mL (n = 6). Alpha diversity for bacterial genera (Chao1, Shannon-Weiner, and Camargo’s evenness indices) were not affected by DIM, meloxicam treatment, parity, or progesterone category. For beta diversity (genera level), principal coordinate analysis (Bray-Curtis) showed differences in microbiota between parity groups. At the phylum level, the relative abundance of Actinobacteria was greater in primiparous than multiparous cows. At the genus level, there was lesser relative abundance of Bifidobacterium, Lactobacillus, Neisseriaceae, Paracoccus, Staphylococcus, and Streptococcus and greater relative abundance of Bacillus and Fusobacterium in primiparous than multiparous cows. Bray -Curtis dissimilarity did not differ by DIM at sampling, meloxicam treatment, or progesterone category at 35 DIM. In conclusion, uterine bacterial composition was not different at 10, 21, or 35 DIM, and meloxicam treatment or progesterone category did not affect the uterine microbiota in clinically healthy postpartum dairy cows. Primiparous cows presented a different composition of uterine bacteria than multiparous cows. The differences in microbiota associated with parity might be attributable to changes that occur consequent to the first calving, but this hypothesis should be investigated further.
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Supplemental Data: Perceived producer barriers to the implementation of best management practices for the transportation of lactating cull cows and control of Salmonella Dublin
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Brunt, Michael; Ritter, Caroline; Renaud, David L.; LeBlanc, Stephen; Kelton, David F. — 2024-03-14 A total of five focus groups involving 28 dairy farmer participants were held in Ontario, Canada from March 14 to April 14, 2023. Twenty-one (75%) participants identified as men and seven (25%) as women. On average, participants were 42 years old (median = 36, range = 20-79) and had 93 lactating cows on their farm (median = 80, range = 40-320). Eighty-two percent of farms (n = 23/28) were freestall, fourteen percent (n = 4/28) were tiestall, and four percent (n = 1/28) were compost-bedded pack.
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Replication Data for: Meta-analysis of the incidence of pregnancy losses in dairy cows at different stages to 90 days of gestation
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Albaaj, Ahmad; Durocher, Jean; LeBlanc, Stephen; Dufour, Simon — 2022-08-05 Results from a literature search using PubMed (https://pubmed.ncbi.nlm.nih.gov/) and CAB (https://www.cabdirect.org/cabdirect) search engines in July 2022 and using “pregnancy OR fetal OR embryonic AND loss OR death” and “dairy AND cattle OR cow OR bos taurus OR bovin” in the title and abstract for PubMed and in the title for CAB to identify articles reporting proportion of pregnancy losses in dairy cows. Comments for inclusion/exclusion of articles are reported.
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Supplemental Data: Producer perspectives on positive welfare opportunities for dairy cows
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Brunt, Michael; Ritter, Caroline; LeBlanc, Stephen; Kelton, David F. — 2024-06-04 A total of five focus groups involving 27 dairy farmer participants were held in Ontario, Canada from March 14 to April 14, 2023. Twenty (74%) participants identified as men and seven (26%) as women. On average, participants were 42 years old (median = 37, range = 20-79) and had 94 lactating cows on their farm (median = 80, range = 40-320). In total, 24 farms were represented by our 27 participants. Eighty-three percent of farms (n = 20/24) were freestall, thirteen percent (n = 3/24) were tiestall, and four percent (n = 1/24) were compost-bedded pack.
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Supplementary data for: A cross-sectional study on antimicrobial use and calf management practices in Canadian pre-weaned dairy calves
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Uyama, Tamaki; Renaud, David L.; Morrison, Emma I.; McClure, J T.; LeBlanc, Stephen; Winder, Charlotte B.; de Jong, Ellen; McCubbin, Kayley D.; Barkema, Herman W; Dufour, Simon; Sanchez, Javier; Heider, Luke C.; Kelton, David F. — 2022-09-09 The supplementary figure shows the questionnaire used in the study. The supplementary tables describe the responses on calf management practices on Canadian dairy farms and univariable analysis of antimicrobial use and farm management practices.

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(Author: LeBlanc, Stephen)

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