
UNB
Carvey, Quinn;
Pavey, Scott A.;
Diamond, Tony;
Davoren, Gail K.;
Lavoie, Raphael A.;
Legard, Matthew;
Robertson, Gregory J.;
Runnells, Emily;
Petalas, Christina;
Major, Heather
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2024-02-20
Blood samples were collected from Atlantic Puffins breeding at five colonies in Atlantic Canada. DNA was extracted using a DNeasy Blood and Tissue DNA Kit from Qiagen. Triple digest genotype-by-sequencing (3D-GbS) library preparation was performed using three restriction enzymes (PstI/NsiI/MspI). Samples were sequenced on one lane using 150 bp, paired-end sequencing with an Illumina NovaSeq 6000. Data were assembled with Stacks v.2.6.1, see publication for full methodological details. Filtered SNP datasets used in genetic analyses are included here.