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Bodnar, Maya; Ratuski, Anna; Weary, D.M 2023-01-18 We investigated the use of isoflurane administered with the drop method at low concentrations prior to carbon dioxide euthanasia. Measures of insensibility and stress-related behaviours were recorded during induction. Our results indicate that the drop method can be used to effectively anesthetize mice with isoflurane concentrations as low as 1.7%; future work should assess mouse aversion.

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