Data were collected in summer 2019, through 3 regions on Southern Québec (Estrie, Laurentides, Outaouais), Canada. For each sampled lake, data were collected for bacterioplankton, phytoplankton, zooplankton and environmental parameters.
Notes: Data for lake name, region, date, latitude (°), longitude (°), maximum depth (m), average depth (m), area (km²), water residence time (year-1), fetch (m);
Lakes were sampled at the deepest point. Data collected for Secchi depth (m) and profiles of: dissolved oxygen (mg/L), temperature (°C), pH, BBE Phycoprobe (µg/L);
Vertical net hauls across water column for crustacean zooplankton: species, size (mm), biomass (µg/L). Vertical tube samples for phytoplankton taxa, size (µm), biovolume (µL/L);
Bottle samples (0.5m depth) were taken from each lake’s deep point for chlorophyll a (µg/L), total nitrogen (mg/L), total phosphorus (µg/L), dissolved organic carbon (mg/L), water color (A440) (m-1) and bacterial 16S metagenomics.