Description:
We show that Ce13d DNAzymes can use non-electrostatic interactions to assist RNA cleavage by making simple Phosphorothioate (PS) modifications at the cleavage junction. In this case, Iodine can replace the role of a metal cofactor, acting directly on the scissile phosphate group. This finding has broadened the scope of DNAzyme catalysis. This DNAzyme was further engineered for fluorescence-based I2 detection with a detection limit of 4.7 nM. In addition, Oxidation of I− with Fe3+ produced I2 in situ, allowing detection of Fe3+ down to 78 nM.
Source Repository:
Water Institute (FRDR)
Publisher(s):
Federated Research Data Repository / dépôt fédéré de données de recherche
License:
Creative Commons Attribution 4.0 International (CC BY 4.0)
https://creativecommons.org/licenses/by/4.0/
URL:
https://doi.org/10.20383/102.0326
Publication date:
2021-03-08
Keywords: