Description:
With the growing consumption of caffeine-containing beverages, detection of caffeine has become an important biomedical, bioanalytical, and environmental topic. Four high-quality aptamers for caffeine were isolated and were carefully characterized. A structure-switching fluorescent sensor was designed showing a detection limit of 1.2 μM caffeine, which reflected the labeled caffeine concentration within 6.1% difference for eight commercial beverages. In 20% human serum, a detection limit of 4.0 μM caffeine was achieved. In addition, these four fluorescent aptamer sensors can form a sensing array, caffeine and the three analogues were well separated from nine other closely related molecules.
Source Repository:
Water Institute (FRDR)
Publisher(s):
Federated Research Data Repository / dépôt fédéré de données de recherche
License:
Creative Commons Public Domain Dedication (CC0 1.0)
https://creativecommons.org/publicdomain/zero/1.0/
URL:
https://doi.org/10.20383/103.0587
Publication date:
2022-06-10
Keywords: