Lipopolysaccharide differentially alters systemic and brain glucocorticoid levels in neonatal and adult mice
- Description:
- We treated male and female C57BL/6J mice at postnatal day 5 (PND5) or PND90 with lipopolysaccharide (LPS; 50 µg/kg bw i.p.) or vehicle and collected blood and brain after 4 hr. We microdissected the prefrontal cortex, hippocampus, hypothalamus, and amygdala. We measured 7 steroids, including corticosterone, via liquid chromatography-tandem mass spectrometry and measured transcripts for key steroidogenic enzymes and hypothalamic-pituitary-adrenal axis components (Cyp11b1, Hsd11b1, Hsd11b2, Crh, Crhr1, Pomc) via qPCR.
- Hamden, Jordan, Salehzadeh, Melody, Bajaj, Hitasha, Li, Michael, and Soma, Kiran
- Contributor(s):
- Hamden, Jordan
- Source Repository:
- UBC Dataverse
- Series:
- UBC Research Data Collection
- Publisher(s):
- Borealis
- Access:
- Public
- License:
- CC-BY 4.0
- URL:
- https://doi.org/10.5683/SP3/QMTF0K
- Publication date:
- 2024-11-18
- Subjects:
- Keywords:
- Identifier:
- https://doi.org/10.5683/SP3/QMTF0K
Geospatial information
There is no geographic information available for this record.
Select geospatial feature(s) to view on map: