Blood samples were collected from Atlantic Puffins breeding at five colonies in Atlantic Canada. DNA was extracted using a DNeasy Blood and Tissue DNA Kit from Qiagen. Triple digest genotype-by-sequencing (3D-GbS) library preparation was performed using three restriction enzymes (PstI/NsiI/MspI). Samples were sequenced on one lane using 150 bp, paired-end sequencing with an Illumina NovaSeq 6000. Data were assembled with Stacks v.2.6.1, see publication for full methodological details. Filtered SNP datasets used in genetic analyses are included here.
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APA Citation:
Carvey, Q., Pavey, S. A., Diamond, T., Davoren, G. K., Lavoie, R. A., Legard, M., Robertson, G. J., Runnells, E., Petalas, C., & Major, H. (2024). Atlantic Puffin (Fratercula arctica) single-nucleotide polymorphism (SNP) datasets [Data set]. University of New Brunswick Dataverse. https://doi.org/10.25545/XWGRBP