Differential sex responses of fathead minnow (Pimephales promelas) gut microbiota to a short-term environmentally-relevant aqueous exposure to benzo[a]pyrene

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Description:
Vertebrate gut microbiota are responsible for regulating several beneficial functions, from development of an organism to maintaining energy homeostasis. However, little is known about the impact of chemical exposures on the structure and function of gut microbiota of fishes. To ascertain a link between contaminant exposure and microbial disruption, male and female fathead minnows (Pimephales promelas) were aqueously exposed to low concentrations of benzo[a]pyrene (BaP) (concentrations ranged from 0.0226 g/L to 1.3 g/L) as well as fish exposed to a solvent control for four days. The samples were sterilely collected from whole fathead minnow guts and stored at -80C until DNA extraction. Gut microbiota were assessed using 16S rRNA metagenetics. Low-level aqueous exposure to BaP resulted in community shifts in bacterial composition in female, but not male, fish. Each fish was analyzed independently (samples were not pooled).This research illustrates that in addition to the well-studied molecular endpoints, community composition of fish gut microbiota can also be impacted by chemical stressors, providing an additional pathway for the generation of adverse effects.
Auteur(s) :
DeBofsky, Abigail

DeBofsky, Abigail

University of Saskatchewan Toxicology Centre
0000-0003-3877-6421
, Xie, Yuwei

Xie, Yuwei

University of Saskatchewan Toxicology Centre
0000-0001-5652-6413
, Grimard, Chelsea

Grimard, Chelsea

University of Saskatchewan Toxicology Centre
, Brinkmann, Markus

Brinkmann, Markus

University of Saskatchewan Toxicology Centre
0000-0002-4985-263
, Hecker, Markus

Hecker, Markus

University of Saskatchewan Toxicology Centre
0000-0002-7237-6192
 et Giesy, John

Giesy, John

University of Saskatchewan Toxicology Centre
0000-0003-1931-9962
Dépôt source:
Global Water Futures (FRDR)
Éditeur(s):
Federated Research Data Repository / dépôt fédéré de données de recherche
License:
Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/
URL:
https://doi.org/10.20383/101.0196
Date de publication:
2024-03-27
Sujets (en):
Biological sciences, microbiome, Microbiology, Microbiome, 16S, sequencing, PAHs, microbial community, fish, freshwater, risk assessment, fish health, fathead minnow, et Pimephales promelas
Identificateur:
https://doi.org/10.20383/101.0196

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Citation selon les normes APA:
Differential sex responses of fathead minnow (Pimephales promelas) gut microbiota to a short-term environmentally-relevant aqueous exposure to benzo[a]pyrene. (2024). [Data set]. Global Water Futures (FRDR). https://doi.org/10.20383/101.0196
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