Multimodal data with wide-field GCaMP imaging and sub-cortical/cortical single unit recording (2015)
Federated Research Data Repository / dépôt fédéré de données de recherche
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2019-07-30
This dataset includes raw wide-field GCaMP image sequences and time stamps of spike trains
simultaneously recorded with GCaMP imaging and processed GCaMP images and spike trains:
df/f0 with 0.1-10 band pass filter and spike train binned in one frame time. Most experiments
were performed toward the end of the mouse light cycle. Mice were anesthetized with isoflurane
(0.5–1.0%) or urethane during data collection. Images of the cortical surface were recorded
through a pair of front-to-front video lenses coupled to a CCD camera. Calcium indicators were
excited with blue-light-emitting diodes (470 nm) with bandpass filters (467–499 nm). Emission
fluorescence was filtered using a 510–550 nm bandpass filter. For in vivo single unit recording,
the electrode was inserted into the right hemisphere with a 45 degree angle from the lateral surface of
the cortex to avoid contact with the region where wide-field imaging was performed. The
electrode was first held in the cortex for cortical recordings and then advanced into the sub-cortical
areas.
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