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PANGAEA Translation missing: fr.blacklight.search.logo
PANGAEA
Vermandele, Fanny; Sasaki, Matthew; Winkler, Gesche; Dam, Hans G; Madeira, Diana; Calosi, Piero 2024 Specimens of the marine calanoid copepod Acartia tonsa were exposed for five days under laboratory conditions to the isolated or combined effects of hypoxia and a marine heatwave event to test for their sex-specific life-history and physiological responses to these stressors. Four treatments were used: control (C: 18 °C, 100 % O₂ sat.), hypoxia (H: 18 °C, 35 % O₂ sat.), marine heatwave (MHW: 25 °C, 100 % O₂ sat.) and combined conditions (HMHW: 25 °C, 35 % O₂ sat.). This dataset compiles all morphological and physiological measurements, namely routine metabolic oxygen consumption rates (µmol h⁻¹ and µmol mg⁻¹ h⁻¹), upper thermal limit (°C) as well as length (mm) and dry weight (µg) following the five-day laboratory exposure experiment. The experiment was conducted in the Marine Ecological and Evolutionary Physiology Laboratory (MEEP) at the University of Quebec in Rimouski (UQAR), Rimouski, QC, Canada. Copepods were maintained following the stock culture culturing protocol described in Dam et al. 2021 (https://doi.org/10.1038/s41558-021-01131-5). Namely, approximately 200 copepods were kept in three 5 L aquaria filled with artificial seawater kept at a salinity of 28.4 ± 0.5. The physicochemical parameters of the seawater were the following: temperature of 18 ± 0.4 °C, oxygen saturation of 93.7 ± 6.0 %, pH NBS of 8.20 ± 0.09. The photoperiod was kept at 13h light: 11 h dark. Copepods were fed ad libitum with a mixture of three phytoplankters: Tetraselmis sp., Thalassiosira weissflogii and Rhodomonas salina. https://creativecommons.org/licenses/by/4.0/legalcode
PANGAEA Translation missing: fr.blacklight.search.logo
PANGAEA
Vermandele, Fanny; Sasaki, Matthew; Winkler, Gesche; Dam, Hans G; Madeira, Diana; Calosi, Piero 2024 Specimens of the marine calanoid copepod Acartia tonsa were exposed for five days under laboratory conditions to the isolated or combined effects of hypoxia and a marine heatwave event to test for their sex-specific life-history and physiological responses to these stressors. Four treatments were used: Control (C: 18 °C, 100 % O₂ sat.), Hypoxia (H: 18 °C, 35 % O₂ sat.), marine heatwave (MHW: 25 °C, 100 % O₂ sat.) and combined conditions (HMHW: 25 °C, 35 % O₂ sat.). This dataset compiles the survival data, expressed in terms of percentage and as the number of individuals at the beginning and end of exposure, for males and females, following the five-day exposure experiment. The experiment was conducted in the Marine Ecological and Evolutionary Physiology Laboratory (MEEP) at the University of Quebec in Rimouski (UQAR), Rimouski, QC, Canada. Copepods were maintained following the stock culture culturing protocol described in Dam et al. 2021 (https://doi.org/10.1038/s41558-021-01131-5). Namely, approximately 200 copepods were kept in three 5 L aquaria filled with artificial seawater kept at a salinity of 28.4 ± 0.5. The physicochemical parameters of the seawater were the following: temperature of 18 ± 0.4 °C, oxygen saturation of 93.7 ± 6.0 %, pH NBS of 8.20 ± 0.09. The photoperiod was kept at 13h light: 11 h dark. Copepods were fed ad libitum with a mixture of three phytoplankters: Tetraselmis sp., Thalassiosira weissflogii and Rhodomonas salina. https://creativecommons.org/licenses/by/4.0/legalcode
PANGAEA Translation missing: fr.blacklight.search.logo
PANGAEA
Vermandele, Fanny; Sasaki, Matthew; Winkler, Gesche; Dam, Hans G; Madeira, Diana; Calosi, Piero 2024 Specimens of the marine calanoid copepod Acartia tonsa were exposed for five days under laboratory conditions to the isolated or combined effects of hypoxia and a marine heatwave event to test for their sex-specific life-history and physiological responses to these stressors. Four treatments were used: Control (C: 18 °C, 100 % O₂ sat.), Hypoxia (H: 18 °C, 35 % O₂ sat.), marine heatwave (MHW: 25 °C, 100 % O₂ sat.) and combined conditions (HMHW: 25 °C, 35 % O₂ sat.). This dataset contains fecundity measurement, represented as the number of offspring (eggs, nauplii and copepodites) spawned by females in each experimental condition following the five-day exposure experiment. The experiment was conducted in the Marine Ecological and Evolutionary Physiology Laboratory (MEEP) at the University of Quebec in Rimouski (UQAR), Rimouski, QC, Canada. Copepods were maintained following the stock culture culturing protocol described in Dam et al. 2021 (https://doi.org/10.1038/s41558-021-01131-5). Namely, approximately 200 copepods were kept in three 5 L aquaria filled with artificial seawater kept at a salinity of 28.4 ± 0.5. The physicochemical parameters of the seawater were the following: temperature of 18 ± 0.4 °C, oxygen saturation of 93.7 ± 6.0 %, pH NBS of 8.20 ± 0.09. The photoperiod was kept at 13h light: 11 h dark. Copepods were fed ad libitum with a mixture of three phytoplankters: Tetraselmis sp., Thalassiosira weissflogii and Rhodomonas salina.----Eggs, offspring and copepodites were retrieved from each aquarium using a 41 µm sieve and placed in a small container. They were then counted one by one under a stereomicroscope (MDF41, Leica) and pipetted in a 500 mL glass beaker. https://creativecommons.org/licenses/by/4.0/legalcode
PANGAEA Translation missing: fr.blacklight.search.logo
PANGAEA
Vermandele, Fanny; Sasaki, Matthew; Winkler, Gesche; Dam, Hans G; Madeira, Diana; Calosi, Piero 2024 Specimens of the marine calanoid copepod Acartia tonsa were exposed for five days under laboratory conditions to the isolated or combined effects of hypoxia and a marine heatwave event to test for their sex-specific life-history and physiological responses to these stressors. Four treatments were used: Control (C: 18 °C, 100 % O₂ sat.), Hypoxia (H: 18 °C, 35 % O₂ sat.), marine heatwave (MHW: 25 °C, 100 % O₂ sat.) and combined conditions (HMHW: 25 °C, 35 % O₂ sat.). This dataset compiles the physico-chemical parameters of the seawater (i.e. temperature, oxygen saturation, pH and salinity) in the aquaria holding the copepods during the five-day exposure experiment. The experiment was conducted in the Marine Ecological and Evolutionary Physiology Laboratory (MEEP) at the University of Quebec in Rimouski (UQAR), Rimouski, QC, Canada. Copepods were maintained following the stock culture culturing protocol described in Dam et al. 2021 (https://doi.org/10.1038/s41558-021-01131-5). Namely, approximately 200 copepods were kept in three 5 L aquaria filled with artificial seawater kept at a salinity of 28.4 ± 0.5. The physicochemical parameters of the seawater were the following: temperature of 18 ± 0.4 °C, oxygen saturation of 93.7 ± 6.0 %, pH NBS of 8.20 ± 0.09. The photoperiod was kept at 13h light: 11 h dark. Copepods were fed ad libitum with a mixture of three phytoplankters: Tetraselmis sp., Thalassiosira weissflogii and Rhodomonas salina. https://creativecommons.org/licenses/by/4.0/legalcode
PANGAEA Translation missing: fr.blacklight.search.logo
PANGAEA
Guscelli, Ella; Noisette, Fanny; Chabot, Denis; Blier, Pierre U; Hansen, Tanya; Cassista-Da Ros, Manon; Pepin, Pierre; Skanes, Katherine R; Calosi, Piero 2024 This study aimed at determining the physiological responses of the northern shrimp, Pandalus borealis, at different levels of biological organization and from four different geographic origins, exposed to elevated temperature and low pH to define its sensitivity to future ocean warming and acidification. Shrimp sampled within the northwest Atlantic were exposed for 30 days to combinations of three temperature (2, 6 or 10°C) and two pH levels (7.75 or 7.40). Survival, metabolic rates, whole-organism aerobic performance and cellular energetic capacity were assessed at the end of the exposure.This dataset is included in the OA-ICC data compilation maintained in the framework of the IAEA Ocean Acidification International Coordination Centre (see https://oa-icc.ipsl.fr). Original data were downloaded from the PANGAEA dataset (see Source). In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2024) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2024-05-13.This project was further supported by:- Strategic Program for Ecosystem- Based Research and Advice grant (SPERA)- Aquatic Climate Change Adaptation Services Program grant (ACCASP)- MITACS-Ouranos Accelerate grant- Réal-Decoste Ouranos scholarship, Award no. 286109 https://creativecommons.org/licenses/by/4.0/legalcode
PANGAEA Translation missing: fr.blacklight.search.logo
PANGAEA
Guscelli, Ella; Chabot, Denis; Vermandele, Fanny; Madeira, Diana; Calosi, Piero 2023 The dataset contains information on the metabolomics reprogramming of the northern shrimp Pandalus borealis collected from four distinct geographic origins (i.e. St. Lawrence Estuary (SLE) 48° 35' N, 68° 35' W, May 2018 ; Eastern Scotian Shelf (ESS), 45° 23' N, 61° 04' W, February 2019 ; Esquiman Channel (EC), 50° 44' N, 57°29' W, July 2019 ; and Northeast Newfoundland Coast (NNC), 50° 18' N, 54° 16' W, November 2019) and exposed for 30 days under laboratory conditions to different ocean global change scenarios of temperature (2, 6, and 10 °C) and pH (7.75 and 7.40), in isolation and in combination. The dataset contains the concentration of key metabolites linked to the aerobic and anaerobic metabolism, expressed as ng metabolite per mg wet weight. Metabolite extraction, identification and quantification was carried out on shrimp muscle tissue by Les laboratoires Iso-BioKem Inc. in February/March 2021. This project was further supported by:1: The DFO Strategic Program for Ecosystem-Based Research and Advice2.The Aquatic Climate Change Adaptation Services Program3. FIR UQAR4. MITACS-Ouranos Accelerate5. The Canada Foundation for Innovation (CFI)6. Réal-Decoste Ouranos scholarship (286109) https://creativecommons.org/licenses/by/4.0/legalcode

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