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Dryad Translation missing: fr.blacklight.search.logo
Peers, Michael; Majchrzak, Yasmine; Menzies, Allyson; Studd, Emily; Bastille-Rousseau, Guillaume; Boonstra, Rudy; Humphries, Murray; Jung, Thomas; Kenney, Alice; Krebs, Charles; Murray, Dennis; Boutin, Stan 2021-06-16 <p style="text-indent:36.0pt;">Canada lynx (<i>Lynx canadensis</i>) and snowshoe hares (<i>Lepus americanus</i>) form a keystone predator-prey cycle that has large impacts on the North-American boreal forest vertebrate community. Snowshoe hares and lynx are both well-suited for snowy winters, but climate change associated shifts in snow conditions could lower hare survival and alter cyclic dynamics. Using detailed monitoring of snowshoe hare cause-specific mortality, behaviour, and prevailing weather, we demonstrate that hare mortality risk is strongly influenced by variation in snow conditions. Although predation risk from lynx was largely unaffected by snow conditions, coyote (<i>Canis latrans</i>) predation increased in shallow snow. Maximum snow depth in our study area has decreased 33% over the last two decades and predictions based on prolonged shallow snow indicate future hare survival could resemble that seen during population declines. Our results indicate that climate change could disrupt cyclic dynamics in the boreal forest.</p>
Trent University Dataverse Translation missing: fr.blacklight.search.logo
Borealis
Wikston, Madison; Wilson, Chris; Murray, Dennis 2023-03-02 MSc research data; raw and managed data for amphibian detections via Visual Encounter Surveys, Larval Dipnet Surveys, Breeding Call Surveys, and qPCR-amplified eDNA samples. Data collected in southern Ontario in 2016. Species-specific synthetic oligonucleotide controls for qPCR are also included.
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Dryad
Prentice, Melanie; Bowman, Jeff; Murray, Dennis; Khidas, Kamal; Wilson, Paul 2020-11-20 Clock genes exhibit substantial control over gene expression and ultimately life-histories using external cues such as photoperiod, and are thus likely to be critical for adaptation to shifting seasonal conditions and novel environments as species redistribute their ranges under climate change. Coding trinucleotide repeats (cTNRs) are found within several clock genes, and may be interesting targets of selection due to their containment within exonic regions and elevated mutation rates. Here, we conduct inter-specific characterization of the NR1D1 cTNR between Canada lynx and bobcat, and intra-specific spatial and environmental association analyses of neutral microsatellites and our functional cTNR marker, to investigate the role of selection on this locus in Canada lynx. We report signatures of divergent selection between lynx and bobcat, with the potential for hybrid-mediated gene flow in the area of range overlap. We also provide evidence that this locus is under selection across Canada lynx in eastern Canada, with both spatial and environmental variables significantly contributing to the explained variation, after controlling for neutral population structure. These results suggest that cTNRs may play an important role in the generation of functional diversity within some mammal species, and allow for contemporary rates of adaptation in wild populations in response to environmental change. We encourage continued investment into the study of cTNR markers to better understand their broader relevance to the evolution and adaptation of mammals.
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Dryad
Vilaca, Sibelle; Grant, Samantha; Beaty, Lynne; Brunetti, Craig; Congram, Megan; Murray, Dennis; Kyle, Christopher 2019-12-04 <p class="BodyA" style="text-indent:36.0pt;margin-bottom:11px;"><a name="_Hlk20505432">Amphibian population declines have been associated with emerging diseases including ranaviruses, which can cause mass die-offs across entire amphibian communities. Understanding and mitigating disease spread requires knowledge of spatial and temporal patterns of pathogen distribution, but also how environmental factors influence pathogen occurrence. We applied environmental DNA (eDNA) detection tools to survey spatial and temporal distributions of ranaviruses by sampling 103 waterbodies in southeastern Ontario, Canada, and assessed the role of abiotic factors as predictors of pathogen occurrence. Ten waterbodies sampled during June-August (&gt;30 km between sites) revealed that ranavirus was marginally more prevalent (<i>p </i>= 0.055) during the latter part of the summer. Ninety-three sites sampled at a finer scale (&lt;10km between sites) exhibited seasonal variability in ranavirus detection (site prevalence: 56% May; 66% July). Occupancy modeling revealed that wetland size and elevation influenced ranavirus<i> </i>occurrence while sampling date and water temperature influenced probability of detection. These findings indicate that biotic factors, such as host density and alternative hosts, should be investigated further as likely determinants of ranavirus prevalence across the landscape. Further, these results highlight the sensitivity of eDNA for detecting widespread presence of ranavirus, and that abiotic factors may have a limited role in determining its prevalence and infectivity.</a></p>

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